International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 6 Issue 6 November-December 2024 Submit your research before last 3 days of December to publish your research paper in the issue of November-December.
Submit Research Paper Join as a Reviewer

Plagiarism is checked by the leading plagiarism checker TurnItIn

Call for Paper

Volume 6 Issue 6
November-December 2024

Submit your research paper

Indexing Partners
Academia Advanced Sciences Index Bielefeld Academic Search Engine CiteSeer DRJI Google Scholar Independent Search Engine & Directory Network ISI (International Scientific Indexing) Issuu Mendeley Research Networks
RefSeek ResearcherId - Thomson Reuters ResearchGate Scirus Scribd Semantic Scholar UTeM - Universiti Teknikal Malaysia Melaka Wiki for Call for Papers WorldCat Zenodo

Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia

Author(s) Grace Mwikuma, Bernard Mudenda Hang'ombe, Francis Musonda, Seke Kazuma, Victor Sichone, Abidan Chansa, Chie Nakajima, Yasuhiko Suzuki
Country Zambia
Abstract This research examined the occurrence of Enterococcus faecalis and Enterococcus faecium in clinical samples processed at Kitwe Teaching Hospital Laboratory, along with their resistance to antimicrobials and presence of virulence determinants. A total of 230 clinical specimens were analyzed to identify suspected Enterococcus strains. Standard laboratory techniques were employed to isolate, characterize, and confirm the species of Enterococcus, followed by testing their susceptibility to antimicrobials and detection of resistance and virulence genes using polymerase chain reaction. Among the 230 cultured specimens, 89 were stool samples, 3 were swabs, and 138 were urine specimens. Out of these, 71 isolates were confirmed Enterococcus by PCR using genus specific primers. The resulting prevalence rate of 30.9% was thus obtained. The most prevalent species was Enterococcus faecalis, accounting for 56.3% of the 71 Enterococcus isolates. The prevalence of E. faecium was 14.1%. All tested E. faecalis isolates displayed resistance to ciprofloxacin and erythromycin. Furthermore, high rates of resistance were observed among E. faecalis isolates for chloramphenicol and tetracycline (97.5%), vancomycin (90.0%), nitrofurantoin (75.0%), penicillin (75.0%) and ampicillin (67.5%). Only two E. faecium isolates exhibited susceptibility to tetracycline and vancomycin, while the remaining isolates were 100% resistant to all other tested antimicrobials. Multidrug resistance was detected in all E. faecium isolates. In addition, various antibiotic resistance genes (aac(6′)-Ie-aph(2″)-LA, ermA, ermB, tetK, tetL, tetM, tetX, vanA) were identified in both E. faecalis and E. faecium isolates. A positive association between phenotype and genotype was found for tetracycline and erythromycin.
Keywords antimicrobial susceptibility, antimicrobial resistance genes, Enterococcus faecalis, Enterococcus faecium, prevalence, clinical specimens, Zambia
Published In Volume 6, Issue 2, March-April 2024
Published On 2024-04-11
Cite This Antimicrobial Resistance Profiles of Enterococcus Faecalis and Enterococcus Faecium Isolated from Clinical Specimens at Kitwe Teaching Hospital in Zambia - Grace Mwikuma, Bernard Mudenda Hang'ombe, Francis Musonda, Seke Kazuma, Victor Sichone, Abidan Chansa, Chie Nakajima, Yasuhiko Suzuki - IJFMR Volume 6, Issue 2, March-April 2024. DOI 10.36948/ijfmr.2024.v06i02.13006
DOI https://doi.org/10.36948/ijfmr.2024.v06i02.13006
Short DOI https://doi.org/gtqx3t
View / Download PDF File

Share this



E-ISSN 2582-2160
CrossRef

CrossRef DOI is assigned to each research paper published in our journal.

IJFMR DOI prefix is
10.36948/ijfmr

Downloads
Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 6 Isu 6Cover Page Vol 6 Isu 5Cover Page Vol 6 Isu 4

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA Open Access

About IJFMR
Fees & Payment
Current Issue
Publication Archive 		Submit Research Paper
Track Submission Status
Publication Guidelines
Publication Ethics
Peer Review & Plagiarism 		Join as a Reviewer
Editors & Reviewers
Reviewer Referral Program
Get Reviewer Membership Certi. 		Website/Journal Policies
Usage Policy
Content Policies
Privacy Policy
Contact Us +91-9898-5948-55 editor@ijfmr.com