Observational Survey to Assess Platelet Concentration and Anti-microbial Potency of Different Blood Extracts

Snigdha Biswas; Rahul Anand; Deba Kumar Das; Arjun Singh; Nagma Raj; Himanshu Kashyap

doi:10.36948/ijfmr.2023.v05i01.1366

Observational Survey to Assess Platelet Concentration and Anti-microbial Potency of Different Blood Extracts

Author(s)	Snigdha Biswas, Rahul Anand, Deba Kumar Das, Arjun Singh, Nagma Raj, Himanshu Kashyap
Country	India
Abstract	Introduction: Platelet concentrates have gained popularity in periodontal regenerative therapy because of its autologous nature. it promotes wound healing after surgical periodontal therapy. Aim: to study the platelet count and antimicrobial efficacy of various platelet concentrates, such as PRP, PRF and IPRF. Methodology: An Observational Study was carried out at Department of Periodontology & Implantology at Babu Banarasi Das College of Dental Sciences. Sample size was 45. 10 ml of blood was drawn from the patient out of which 3 ml each of blood will be used for PRP, PRF and I-PRF preparation and remaining 1 ml of blood was used for determining the platelet count. For I-PRF preparation, 3 ml of blood was used, d centrifuged at 700 rpm for 3 minutes. For PRF preparation, Agar plates will be inoculated with plaque sample of same patient and will be labelled and divided into 3 compartments. Wells was prepared in the inoculated agar plate and 0.1 ml of PRP, PRF and I-PRF will be placed in those wells. Inoculated blood agar plates was then incubated aerobically at 37° C for 24 hours to 48 hours. Results: The platelet count of i-PRF was statistically significant when compared to control (P < 0.001). It was also significant when compared to PRP (P < 0.01) and PRF (P < 0.001). Mean zone of inhibition around i-PRF (P < 0.01) and PRF (P < 0.05) reached statistical significance. Although a distinct zone of inhibition was seen with PRP, it was not statistically significant (P > 0.05). Conclusion: In the present study, although a distinct zone of inhibition was obtained with all test samples, it was significant with only i-PRF and PRF.
Keywords	Platelet Concentrates, Antimicrobial Efficacy, PRP, PRF, IPRF
Field	Medical / Pharmacy
Published In	Volume 5, Issue 1, January-February 2023
Published On	2023-01-11
Cite This	Observational Survey to Assess Platelet Concentration and Anti-microbial Potency of Different Blood Extracts - Snigdha Biswas, Rahul Anand, Deba Kumar Das, Arjun Singh, Nagma Raj, Himanshu Kashyap - IJFMR Volume 5, Issue 1, January-February 2023. DOI 10.36948/ijfmr.2023.v05i01.1366
DOI	https://doi.org/10.36948/ijfmr.2023.v05i01.1366
Short DOI	https://doi.org/grpdq4

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Observational Survey to Assess Platelet Concentration and Anti-microbial Potency of Different Blood Extracts

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