Studies of Erk1/2 with Respect to Tumor Aggressiveness in B16f10 Murine Melanoma Cells

Shuvojit Moulik; Sayantani Karmakar; Asmita Basu; Swayambhik Mukherjee; Neeraja R.; Amitava Chatterjee

doi:10.36948/ijfmr.2023.v05i01.1733

Studies of Erk1/2 with Respect to Tumor Aggressiveness in B16f10 Murine Melanoma Cells

Author(s)	Shuvojit Moulik, Sayantani Karmakar, Asmita Basu, Swayambhik Mukherjee, Neeraja R., Amitava Chatterjee
Country	India
Abstract	Melanoma is a very aggressive form of skin cancer, it has the potential to spread from a small sized primary tumor and metastasizes to different locations like lungs, bones, lymph nodes, liver and even brain. Extracellular signal regulated kinase (ERK) molecule present in MAPK pathway plays a major role in different carcinogenic processes like cell migration, cell invasiveness by altering the extracellular matrix (ECM). Cell invasiveness is facilitated by matrix metalloproteins (MMPs) by means of degradation of gelatinase enzyme regulated by a family of receptor proteins called integrins. ECM ligand fibronectin causes increased secretion of MMPs, specially MMP2 and MMP9 facilitating invasiveness and metastasis in cancer cells. This study aims to find the modulatory role of ERK1/2 with respect to tumor aggressiveness on both MMP2 and MMP9 in context of melanoma metastasis and invasion. ERK pathway was downregulated through administration of inhibitors. ERK1/2 siRNA gene silencing the effect on MMP2 and MMP9 was studied. The studies were done in B16F10 melanoma cell line and mice model. Significant changes were found in levels of MMP2 and MMP9 after downregulating the ERK pathway and therefore a correlation between ERK and MMP activities was established. Elucidating these molecular phenomena in melanoma, this result can help in developing and improving targeted cancer therapies with multiple options in drug discovery.
Keywords	Melanoma, ERK, Extracellular Matrix, MAPK, Matrix Metalloprotease, Integrins
Field	Biology > Genetics / Molecular
Published In	Volume 5, Issue 1, January-February 2023
Published On	2023-02-28
Cite This	Studies of Erk1/2 with Respect to Tumor Aggressiveness in B16f10 Murine Melanoma Cells - Shuvojit Moulik, Sayantani Karmakar, Asmita Basu, Swayambhik Mukherjee, Neeraja R., Amitava Chatterjee - IJFMR Volume 5, Issue 1, January-February 2023. DOI 10.36948/ijfmr.2023.v05i01.1733
DOI	https://doi.org/10.36948/ijfmr.2023.v05i01.1733
Short DOI	https://doi.org/grwbgz

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Studies of Erk1/2 with Respect to Tumor Aggressiveness in B16f10 Murine Melanoma Cells

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