Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples

Shveta Tiwari; Varsha Shukla; Anushree Lokur

doi:10.36948/ijfmr.2024.v06i02.17549

Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples

Author(s)	Shveta Tiwari, Varsha Shukla, Anushree Lokur
Country	India
Abstract	Pathogens emerge and spread from a variety of sources including food, water, air, and clinical samples, resulting in disease epidemics in the community. The early detection of pathogens in food and water samples can help prevent illness transmission. Therefore, detection of harmful bacteria is vital in this setting. Food and water-borne illnesses are more common in underdeveloped and emerging countries than in affluent countries. Because of the lack of well-equipped centralized laboratory facilities, many cases are unreported, resulting in an underestimation of the bioburden of the disease. To address the limitations of culture-based approaches, infections are discovered using advanced methods such as nucleic acid or immunological methods, which are costly and require extensive sample preparation. This study focused on the development of a paper-based Portable Culture Device (PCD) capable of detecting Listeria monocytogenes. The device, optimized at a substrate concentration of 3 gL-1 and a temperature range of 28°C-37°C, can detect cell counts of up to 10^6 CFU ml^-1. The development of paper-based microfluidic detection devices has the potential to overcome the limitations of current organism-detection technologies. These devices adhere to ASSURED standards, which require them to be inexpensive, sensitive, user-friendly, quick, resilient, equipment-free, and supplied to the needy. This could help solve many of the issues associated with centralized testing facilities and simplify on-site microbe detection.
Keywords	On-site detection, foodborne pathogens, Listeria monocytogenes, Point of care testing (POCT), Portable Culture Device (PCD)
Published In	Volume 6, Issue 2, March-April 2024
Published On	2024-04-17
Cite This	Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples - Shveta Tiwari, Varsha Shukla, Anushree Lokur - IJFMR Volume 6, Issue 2, March-April 2024. DOI 10.36948/ijfmr.2024.v06i02.17549
DOI	https://doi.org/10.36948/ijfmr.2024.v06i02.17549
Short DOI	https://doi.org/gtrfk9

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Standardization of Portable Culture Device for Detection of Listeria Monocytogenes from Food Samples

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