Isolation of Thermophilic Bacteria in Hot Spring of Asin, Tuel, Tublay, Benguet

Author(s)	Jayceie P. Salming
Country	Philippines
Abstract	Background and Objectives: Thermophilic bacteria are less studied but they are important group of microorganisms because of their ability to produce industrial enzymes. Materials and Methods: In this study, thermophilic bacteria were isolated from hot spring of Asin, Tuba, Benguet. A bacterium that could tolerate high temperatures was characterized by morphology, biochemistry and sequencing of its 16S rRNA gene. The isolate was screened for protease and amylase activity. Phylogenetic affiliations of the isolate was studied. Results: The bacterium with the ability to tolerate high temperatures was identified as Bacillus sp. both by morphology, biochemistry and sequencing of its 16S rRNA gene. BLAST search analysis of the sequence showed maximum identity with Bacillus sp (99% and 100% similarity). Phylogenetic analysis of the isolate revealed close affiliation with thermophilic Bacillus species. Conclusion: The study confirmed that the isolated Bacillus sp. to be a true thermophile and could be a source of thermostable amylase which can be exploited for food industries.
Field	Biology
Published In	Volume 6, Issue 3, May-June 2024
Published On	2024-06-10
Cite This	Isolation of Thermophilic Bacteria in Hot Spring of Asin, Tuel, Tublay, Benguet - Jayceie P. Salming - IJFMR Volume 6, Issue 3, May-June 2024. DOI 10.36948/ijfmr.2024.v06i03.20911
DOI	https://doi.org/10.36948/ijfmr.2024.v06i03.20911
Short DOI	https://doi.org/gtzjqr

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