International Journal For Multidisciplinary Research

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Production and Characterization of Biologically Important Red Pigment (PRODIGIOSIN) Produced by Serratia Marcescens

Author(s) Anjali Rani, Rupali Kumari, Dr. Vinod kumar gupta
Country India
Abstract The study was aimed to formulate and evaluate cream from prodigiosin extract from Serratia marcescens. Gram staining technique revealed the bacterium's cell wall structure. By analysing the behaviour of S. marcescens in a hanging drop preparation, determined it’s motility and presence and type of flagellar it possesses. Endospore staining illuminated the presence or absence of endospores within the bacterial population. From Endospore staining concluded that S. marcescens does not produce endospores. Biochemical tests such as:- Catalse, Urease, Citrate, Nitrate reduction, MR-VP (Methyl Red–Voges Proskauer), Indole, etc.revealed key metabolic pathways and enzymatic activities. The oxidative-fermentative test provided insights into its metabolic versatility. Antibiotic susceptibility testing (AST) elucidated its response to antibiotic. Furthermore, fermentation media preparation and growth optimization growth optimization techniques were explored to enhance culture conditions at different temperature, pH and carbon source. TLC analysis showed a single band with an Rf value of 0.62. Prodigiosin has a characteristic absorption peak in the visible region of spectrum typically around 500- 550nm. Moreover, a cream formulation was developed, incorporating S. marcescens pigments called Prodigiosin with subsequent antibiotic susceptibility testing to evaluate its potential therapeutic efficacy. After that evaluation of cream was done. pH of formulated cream was 6, no skin irritation, homogeneity was good and AST test of cream showed zone of inhibition against S.aureus. This multidisciplinary approach offers a comprehensive understanding of S. marcescens and it provides valuable insights for clinical and pharmaceutical applications.
Keywords Serratia marcescens, Gram’s staining, hanging drop motility, Endospore staining, biochemical analysis, Antibiotic Susceptibility test, Fermentation media, Growth optimisation., Cream Formulation
Published In Volume 6, Issue 3, May-June 2024
Published On 2024-05-29
Cite This Production and Characterization of Biologically Important Red Pigment (PRODIGIOSIN) Produced by Serratia Marcescens - Anjali Rani, Rupali Kumari, Dr. Vinod kumar gupta - IJFMR Volume 6, Issue 3, May-June 2024. DOI 10.36948/ijfmr.2024.v06i03.21324
DOI https://doi.org/10.36948/ijfmr.2024.v06i03.21324
Short DOI https://doi.org/gtw6sj
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