Physico - Chemical Characterization of Alkaline Protease Produce by Bacillus Sp

Author(s)	Jariwala Janvi N, Langalia Yash N, Parekh Sanjay N
Country	India
Abstract	In this study, Alkaline protease producing bacterial strains were isolated from various sample using skimmed milk agar. Among the isolates, Strain RE-1 shows highest protease activity which was identified as Bacillus sp. by partial 16S rRNA sequencing. The isolate showed maximum alkaline protease production (89.396 U/ml) at pH 8.0 and 30°C within 24 h of incubation. The enzyme exhibited thermal stability in the range of 40 to 70oC. Specific activity of partially purified enzyme is found to be 0.53 U/mg with 37.8% yield.
Keywords	Alkaline protease, Enzyme Assays, 16S rDNA, Industrial effluent.
Field	Biology > Bio + Chemistry
Published In	Volume 6, Issue 3, May-June 2024
Published On	2024-06-06
Cite This	Physico - Chemical Characterization of Alkaline Protease Produce by Bacillus Sp - Jariwala Janvi N, Langalia Yash N, Parekh Sanjay N - IJFMR Volume 6, Issue 3, May-June 2024. DOI 10.36948/ijfmr.2024.v06i03.21347
DOI	https://doi.org/10.36948/ijfmr.2024.v06i03.21347
Short DOI	https://doi.org/gtxrr5

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