International Journal For Multidisciplinary Research

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Androgenesis in Hot Pepper by Chilli Anther Culture

Author(s) Komal Purushottam Kharad, Sarosha Khan
Country India
Abstract In vitro androgenesis techniques play a key role in the quick generation of fully homozygous lines. This work's goal was to put the procedure for producing androgenic embryos in hot pepper populations into practice. A number of variables can affect androgenetic success, including the types and quantities of carbohydrate sources and the nature of the nutritional medium. Different sugar types were tested at different doses in anther culture of pepper to elucidate their influence on the yield of androgenesis. In this study, sucrose was used as a source of sugar, Murashige & Skoog (1962-MS) medium, silver nitrate (AgNO3), naphthaleneacetic acid (NAA), BAP and one breeding lines of pepper were used to know the genotypic effects on haploid embryo formation. It was found that one of the key elements influencing pepper anther and microspore culture success is genotype. Also, microspore stage and the cold treatment of flower bud responsible for the success of embryo formation. This embryo formation frequency has been calculated according to the results that were generated in one month and the frequency of embryo formation is 0.7%.
Keywords Androgenesis, chilli anther culture, haploid plant production
Field Biology > Agriculture / Botany
Published In Volume 6, Issue 3, May-June 2024
Published On 2024-06-27
Cite This Androgenesis in Hot Pepper by Chilli Anther Culture - Komal Purushottam Kharad, Sarosha Khan - IJFMR Volume 6, Issue 3, May-June 2024. DOI 10.36948/ijfmr.2024.v06i03.22426
DOI https://doi.org/10.36948/ijfmr.2024.v06i03.22426
Short DOI https://doi.org/gt245m
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