In Silico Analysis and Validation of ymcA Gene of Bacillus Subtilis

Author(s)	BINAL RAHATE, PARAMJITKAUR KHAIRA
Country	India
Abstract	This study aimed to analyze and validate the ymcA gene of Bacillus subtilis in silico. The bacterial strains used were B. subtilis 2049 (T) and B. subtilis HS, obtained from the National Center for Microbial Resource, Pune. The media used were LB medium and Mineral Salt Medium (MSM). The biofilm formation assay was performed in a microtiter plate, with each well inoculated with 980 µl of MSM and 20 µl of test cultures. The plate was incubated for 120 hrs at 37°C following phosphate buffer assay. The contents of the plate were removed by gently tapping at the bottom of the plate, rinsed with phosphate buffer saline, and de-stained using 70% ethanol for 15 min. The genomic DNA of B. subtilis 2049 (T) was extracted using the Chloroform: Isoamylalcohol method. The supernatant was taken in a sterile tube and stored at -80°C until further use.
Keywords	In Silico Analysis, ymcA Gene, Basillus subtilis
Field	Biology
Published In	Volume 6, Issue 3, May-June 2024
Published On	2024-06-23
Cite This	In Silico Analysis and Validation of ymcA Gene of Bacillus Subtilis - BINAL RAHATE, PARAMJITKAUR KHAIRA - IJFMR Volume 6, Issue 3, May-June 2024. DOI 10.36948/ijfmr.2024.v06i03.22773
DOI	https://doi.org/10.36948/ijfmr.2024.v06i03.22773
Short DOI	https://doi.org/gt245c

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