International Journal For Multidisciplinary Research

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Characterization of Yeast isolates NB1 and NB3 from ITS Region of the Leaf of Alstonia Scholaris

Author(s) Sonali Chib
Country India
Abstract For experimental study , refrigeration stored stock culture of two isolated novel yeast strains namely NB1 and NB3 were used . These isolates were freshly grown at pH 5.8 and 30°C temperature for culture revival. For the characterization of isolated yeast strains; genomic DNA isolation and identification of strains using ITS primer ITS 1 and ITS 4 through PCR reaction was conducted. Growth curve analysis of isolated (NB1 and NB3) yeast strain shown that both the isolated yeast strains (NB1 and NB3) revealed varying growth pattern, both of them were faster growing strains with short life span. For phenotypic characterization: growth parameters, morphological and physiochemical characterization was carried out as Hydrogen peroxide test, Thermo tolerance test, Glycerol test, Galactose test , Growth at different temperature , Methyl Methane Sulphonate and pH of the isolated yeast strains , was performed. All these tests revealed that the isolated yeast strains were highly resistant at different pH conditions on YPD agar medium ( pH= 5.8,7,8), at higher concentration of H2O2, Glycerol, Galactose reveals tolerance but in case of MMS drug concentration shows little tolerance when spotted with control BY474 1 Reference laboratory strain on YPD agar plates with incubation at 30°C for 48 hours.
Keywords yeast peptone dextrose , ITS , Yeast, media
Field Biology > Genetics / Molecular
Published In Volume 6, Issue 5, September-October 2024
Published On 2024-09-10
Cite This Characterization of Yeast isolates NB1 and NB3 from ITS Region of the Leaf of Alstonia Scholaris - Sonali Chib - IJFMR Volume 6, Issue 5, September-October 2024. DOI 10.36948/ijfmr.2024.v06i05.27301
DOI https://doi.org/10.36948/ijfmr.2024.v06i05.27301
Short DOI https://doi.org/gwfgjg
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