International Journal For Multidisciplinary Research

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Impact of Molecular Identification of Palm leaves on the preservation and conservation of Palm Leaves-Tala patra Manuscripts

Author(s) Ashwini M, Ramakrishna Bhat K
Country India
Abstract Palm leaves manuscripts preservation is the main target of this research work. Therefore, this research study aimed at molecular identification of palm leaves by DNA Barcoding to assess the potential level on manuscript preservation. This study aims to amplify the rbcL and matK genes in palm leaves collected from different regions of Bangalore. The DNA was isolated by CTAB method and the amplification was done by PCR analysis. DNA barcoding proved to be an efficient tool for proper discrimination of the related species by generating standers that can be used universally. rbcL: A gene found in the chloroplast genome, encoding a large subunit of the enzyme ribulose-bisphosphate carboxylase. matK: Another chloroplast gene coding for maturase K, involved in splicing group II introns.
The discrimination is done by accurately sequencing the standard gene region in a very short time (Hebert et al., 2003). rbcL and matK have been having most recommended and vasty studied DNA barcodes for plants, as they are proved by PWG (Pair wise group) to be potential standardized barcodes for plant DNA barcoding. These barcodes are used to properly identify and discriminate any native or foreign plant species (Maloukh et al., 2017). This work guides us the impact/role of molecular identification-DNA Barcoding of palm leaves on the preservation of Palm leaves manuscripts
Keywords Barcoding Polymerase chain reaction rbcL matK
Field Biology > Genetics / Molecular
Published In Volume 6, Issue 5, September-October 2024
Published On 2024-09-13
Cite This Impact of Molecular Identification of Palm leaves on the preservation and conservation of Palm Leaves-Tala patra Manuscripts - Ashwini M, Ramakrishna Bhat K - IJFMR Volume 6, Issue 5, September-October 2024. DOI 10.36948/ijfmr.2024.v06i05.27492
DOI https://doi.org/10.36948/ijfmr.2024.v06i05.27492
Short DOI https://doi.org/gzwpz3
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