Isolation of Tyrosinase Producing Bacteria from Rhizosphere Soil Samples

Author(s)	Barate D. L., Sonar N. R.
Country	India
Abstract	The present study is based on the isolation and identification of the bacteria producing tyrosinase enzyme which play an important role in production of L-DOPA amino acid which is used to treat Parkinson’s disease. In the study, 42 rhizosphere soil samples were collected from Akola and Amravati district, out of which 15 isolates were found to be tyrosinase enzyme producers when screened on tyrosine agar medium. Two isolates NT and ST showed most prominent tyrosinase production which latter checked for the effect of parameters on tyrosinase activity. Results revealed that tyrosinase activity was optimum at temperature range 37℃ - 42°C, pH 6 for NT isolate and pH 9 for ST isolate and time course 72 hours. Further effect of nitrogen sources showed prominent increase in tyrosinase activity after addition of 1% sodium and potassium nitrate while addition of carbon sources did not show any remarkable increase in tyrosinase activity.
Keywords	Tyrosinase, L-DOPA, L-Tyrosine, Rhizosphere
Published In	Volume 5, Issue 3, May-June 2023
Published On	2023-05-04
Cite This	Isolation of Tyrosinase Producing Bacteria from Rhizosphere Soil Samples - Barate D. L., Sonar N. R. - IJFMR Volume 5, Issue 3, May-June 2023. DOI 10.36948/ijfmr.2023.v05i03.2787
DOI	https://doi.org/10.36948/ijfmr.2023.v05i03.2787
Short DOI	https://doi.org/gr7hdr

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