Micropropagation of an Arid Zone Leguminous Tree – Acacia Senegal (L.) Willd Via Callus

Author(s)	Parul Gupta
Country	India
Abstract	An efficient micropropagation system was developed for an economically important arid zone tree species-Acacia senegal (L.) Willd., commonly known as Gum Arabic. Callus induction and high frequency regeneration was obtained on Murashige and Skoog (MS) medium along with auxins, (NAA, 2,4-D, IAA), cytokinins (BAP, Kn) and additives like AA and AS. Rooting of the regenerated shoots was initiated on ½ MS-medium + NAA (2.5 mgl-1) + IBA (2.5 mgl-1) and low sucrose level (3%). However, further elongation and development of roots was achieved on ¼ MS + IBA (0.5 mgl-1) and still lower sucrose level (1%).For the hardening and acclimatization , the in vitro regenerated plantlets were transferred to a sterile vermiculite and sand mixture in a 3:1 ratio. Subsequently the hardened plantlets were transferred to a green house and finally to the fields.
Keywords	Micropropagation, Acacia senegal, Callus, differentiation
Field	Biology > Agriculture / Botany
Published In	Volume 3, Issue 6, November-December 2021
Published On	2021-11-08
Cite This	Micropropagation of an Arid Zone Leguminous Tree – Acacia Senegal (L.) Willd Via Callus - Parul Gupta - IJFMR Volume 3, Issue 6, November-December 2021.

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