International Journal For Multidisciplinary Research

E-ISSN: 2582-2160   •   Impact Factor: 9.24

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 7, Issue 2 (March-April 2025) Submit your research before last 3 days of April to publish your research paper in the issue of March-April.
Submit Research Paper Join as a Reviewer

Plagiarism is checked by the leading plagiarism checker TurnItIn

Call for Paper

Volume 7 Issue 2
March-April 2025

Submit your research paper

Indexing Partners
Academia Advanced Sciences Index Bielefeld Academic Search Engine CiteSeer DRJI Google Scholar Independent Search Engine & Directory Network ISI (International Scientific Indexing) Issuu Mendeley Research Networks
RefSeek ResearcherId - Thomson Reuters ResearchGate Scirus Scribd Semantic Scholar UTeM - Universiti Teknikal Malaysia Melaka Wiki for Call for Papers WorldCat Zenodo

An Integrated Next Generation Sequence Analysis of Blood Cancer Along with Biopython

Author(s) Ms. Kiranmai Venkatagiri, Uma Kumari
Country India
Abstract Blood cancers, including leukaemia, lymphoma, and myeloma, are among the most prevalent haematological malignancies. PDE6D, a key regulator of oncogenic RAS signalling, has been implicated in cancer progression. This study integrates Next-Generation Sequencing (NGS) with computational bioinformatics to analyse PDE6D mutations and their role in blood cancer pathogenesis. The 7PAC protein structure (PDE6D) was retrieved from the Protein Data Bank and analysed using PyMOL for structural visualization. Sequence alignment and homology studies were conducted using BLAST and Cluster Omega. Root Mean Square Deviation (RMSD) analysis assessed structural variations, while protein enrichment analysis and pathway analysis explored PDE6D’s role in oncogenic signalling. B-factor analysis identified dynamic regions susceptible to mutation. BioPython was employed for genomic data analysis and functional annotation. STRING analysis revealed significant PDE6D interactions, particularly with RPGR, suggesting its involvement in cellular processes. KEGG pathway analysis confirmed its role in RAS-driven leukaemia. Root mean square analysis indicated high structural conservation, while B-factor analysis highlighted mutation-prone regions. Bioinformatics analysis suggested PDE6D mutations may contribute to dysregulated RAS signalling in blood cancers. This study highlights PDE6D as a potential biomarker and therapeutic target in blood cancer. By integrating NGS with bioinformatics tools, we identified critical genetic variations affecting its oncogenic role. Further in vitro and in vivo studies are required to validate PDE6D-targeted therapies, advancing precision medicine for blood cancer treatment.
Keywords NGS; Structure Analysis; Blood cancer; BioPython; Proteomics; Mutations,Sequence Alignment
Field Biology > Genetics / Molecular
Published In Volume 7, Issue 2, March-April 2025
Published On 2025-03-24
DOI https://doi.org/10.36948/ijfmr.2025.v07i02.39648
Short DOI https://doi.org/g89v67
View / Download PDF File

Share this



E-ISSN 2582-2160
CrossRef

CrossRef DOI is assigned to each research paper published in our journal.

IJFMR DOI prefix is
10.36948/ijfmr

Downloads
Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 7 Isu 2Cover Page Vol 7 Isu 1Cover Page Vol 6 Isu 6

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA Open Access

About IJFMR
Fees & Payment
Current Issue
Publication Archive 		Submit Research Paper
Track Submission Status
Publication Guidelines
Publication Ethics
Peer Review & Plagiarism 		Join as a Reviewer
Editors & Reviewers
Reviewer Referral Program
Get Reviewer Membership Certi. 		Website/Journal Policies
Usage Policy
Content Policies
Privacy Policy
Contact Us +91-9687-828-838 editor@ijfmr.com