Isolation and Identification of Complex Microbiota from Milk and Milk Products and Anti-Microbial Activity of Fruit Peels Against Isolated Bacteria

Omkar Mishra; Dharitri Priyadarsini; Pavithra S; Rajat Kumar Nayak

doi:10.36948/ijfmr.2023.v05i04.4507

Isolation and Identification of Complex Microbiota from Milk and Milk Products and Anti-Microbial Activity of Fruit Peels Against Isolated Bacteria

Author(s)	Omkar Mishra, Dharitri Priyadarsini, Pavithra S, Rajat Kumar Nayak
Country	India
Abstract	Milk and milk products are highly nutritious food source that can support growth of many complex microbiota. The development of various culture techniques helps us in isolating and culturing such microorganisms from milk and milk products. Gram staining was used as a preliminary approach. Initially, bacterial identification was done by targeting the cell wall composition by gram staining. Further to identify the genus and species of bacteria different biochemical tests were conducted. These microorganisms enter from a variety of sources and can play a number of roles like facilitating dairy fermentations (e.g., Streptococcus, Lactobacillus), spoilage (e.g., Clostridium, Pseudomonas), causing disease (e.g., Salmonella spp., E. coli). The growth of these causative organisms can be inhibited by the implementation of various anti-microbial tests. The microbial inhibition properties inflicted by voluntarily selected fruit peel extracts was studied against the identified disease-causing microbes. Fruit peels that are being left out after the fruit extract is processed out, are generally rich in bioactive compounds like polyphenols and carotenoids, exhibiting antimicrobial, antioxidant, and anti-cancer properties. The anti-oxidants present in the peels of citrus fruits act against the growth of spoilage and disease-causing microbes.
Keywords	Dairy product, polyphenol, carotenoid, antimicrobial activity, Vibrio, Staphylococcus, Pseudomonas
Field	Biology > Bio + Chemistry
Published In	Volume 5, Issue 4, July-August 2023
Published On	2023-07-20
Cite This	Isolation and Identification of Complex Microbiota from Milk and Milk Products and Anti-Microbial Activity of Fruit Peels Against Isolated Bacteria - Omkar Mishra, Dharitri Priyadarsini, Pavithra S, Rajat Kumar Nayak - IJFMR Volume 5, Issue 4, July-August 2023. DOI 10.36948/ijfmr.2023.v05i04.4507
DOI	https://doi.org/10.36948/ijfmr.2023.v05i04.4507
Short DOI	https://doi.org/gshm7t

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Isolation and Identification of Complex Microbiota from Milk and Milk Products and Anti-Microbial Activity of Fruit Peels Against Isolated Bacteria

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