International Journal For Multidisciplinary Research

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Screening and Characterization of Maecescens Serretia and Proteus Myxofaciens from Soil Sample

Author(s) Sunidhi Sharma, Damini, Vinod Kumar Gupta
Country India
Abstract The aim of current study was to identify, characterize and isolate protease producing bacteria from soil sample. Commencing from the isolation by spreading technique on nutrient agar media, at 350C for 20 hrs. Followed by screening and characterization based on temperature, pH, nitrogen, and carbon sources. Two colonies U-2 and U-4 were selected and identified as Maecescens Serretia and Proteus Myxofaciens, the highest urease producing bacteria among the four that were taken forward for the study. Different Biochemical tests and microscopic identification tests were done for the screening of the bacteria. The U-2 bacteria showed maximum growth at temperature 350C, pH needed 3, potassium nitrate as nitrogen source and d-mannitol as carbon sources. Whereas, temperature requirement for the highest growth for U-4 was 250C, dextrose as carbon source but pH and nitrogen source were like the U-2 bacteria. At last, enzyme activity assay was performed to know the urease activity using well diffusion method.
Keywords Characterization, Maecescens Serretia, Proteus Myxofaciens, diffusion, enzyme activity.
Field Biology > Bio + Chemistry
Published In Volume 5, Issue 5, September-October 2023
Published On 2023-10-27
Cite This Screening and Characterization of Maecescens Serretia and Proteus Myxofaciens from Soil Sample - Sunidhi Sharma, Damini, Vinod Kumar Gupta - IJFMR Volume 5, Issue 5, September-October 2023. DOI 10.36948/ijfmr.2023.v05i05.7935
DOI https://doi.org/10.36948/ijfmr.2023.v05i05.7935
Short DOI https://doi.org/gszvh8
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