International Journal For Multidisciplinary Research

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Thermal Inactivation Kinetics and Inhibition Studies of Watermelon (Citrullus vulgaris) Seeds Urease

Author(s) Dr. Samarpita Gupta
Country India
Abstract Urease was isolated from the seeds of watermelon (Citrullus vulgaris). The optimum temperature and pH was found to be 50° C and 8.3 respectively. Urease shows no loss in their activity when treated overnight with assay buffer of different pH. Watermelon urease thermal inactivation studies revealed a variation in kinetic pattern at different temperatures. At temperature of 50° C and 60° C, urease shows almost no loss in activity i.e. their stable nature. Thermal inactivation (time dependent) shows biphasic kinetics at 70° C, 80° C and 85° C. Investigation studies strongly support the oligomeric nature of the enzyme. The Km and Vmax of Citrullus vulgaris urease was found to be 10 mM and 142.85 µmole of urea/min/mg of protein. The plot of percent residual activity versus the number of days gave a t1/2 of 70 days for urease stored at 4° C and 16 days for urease that stored at 37° C. The I50 values for Hg2+ and NaCl was found to be 6.9 nM and 240 mM respectively, which shows strong inhibitory nature of Hg2+.
Keywords Urease, Watermelon, Kinetics, Inhibition, Citrullus Culgaris
Field Biology > Agriculture / Botany
Published In Volume 4, Issue 5, September-October 2022
Published On 2022-10-31
Cite This Thermal Inactivation Kinetics and Inhibition Studies of Watermelon (Citrullus vulgaris) Seeds Urease - Dr. Samarpita Gupta - IJFMR Volume 4, Issue 5, September-October 2022. DOI 10.36948/ijfmr.2022.v04i05.899
DOI https://doi.org/10.36948/ijfmr.2022.v04i05.899
Short DOI https://doi.org/grb57j
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