International Journal For Multidisciplinary Research

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Anti-Mitotic Activity Of Gumamela (Hibiscus Rosa-Sinensis) Leaf Extract In The Early In-Vitro Development Of Sea Urchin (Tripneustes Gratilla) Embryo

Author(s) Nowaini Makabinta, Genevieve Tonog, Mohammad Sadic Usman, Vincent Bergado
Country Philippines
Abstract Plant-derived products have played an important role in the development of several clinically useful anticancer agents. Several cancer treatments have been widely used, including surgery, chemotherapy, and radiotherapy. However, no selective cancer therapies exist that can eliminate cancer cells while preserving healthy cells unharmed. This study aims to determine the antimitotic activity of Hibiscus rosa-sinensis leaf extract in the early in-vitro development of sea urchin Tripneustes gratilla embryo.

The Hibiscus rosa-sinensis leaves were extracted using ethanol into four different concentrations (0.5%, 1.0%, 1.5%, 2.0%). A Sea urchin bioassay was then performed to determine its antimitotic activity against the Tripneustes gratilla embryo. Phytochemical confirmatory tests were also done to confirm the presence of bioactive compounds. The results of the study showed that Hibiscus rosa-sinensis leaf extracts contained alkaloids, flavonoids, tannins, and saponins.

The Sea Urchin Bioassay reveals that the various leaf extract concentrations (0.5%, 1.0%, 1.5%, 2.0%) inhibited antimitotic activity against the early cell development of sea urchin embryo as it had a slower rate of mitotic activity as compared to the negative control. Moreover, the higher concentration of leaf extract 1.5% and 2.0% revealed to have greater antimitotic activity similar to that of the positive control colchicine as data showed it had the least number of embryos developed. This implies that the antimitotic activity of Hibiscus rosa-sinensis leaf extracts is concentration-dependent.

An ANOVA test showed that there are significant differences between the various leaf concentrations and control groups in the early cell development of sea urchin embryos in minute intervals.
Keywords Anti-mitotic activity, In-vitro Development, Inhibition, Phytochemicals, Sea Urchin Bioassay
Field Medical / Pharmacy
Published In Volume 6, Issue 5, September-October 2024
Published On 2024-10-28
Cite This Anti-Mitotic Activity Of Gumamela (Hibiscus Rosa-Sinensis) Leaf Extract In The Early In-Vitro Development Of Sea Urchin (Tripneustes Gratilla) Embryo - Nowaini Makabinta, Genevieve Tonog, Mohammad Sadic Usman, Vincent Bergado - IJFMR Volume 6, Issue 5, September-October 2024. DOI 10.36948/ijfmr.2024.v06i05.28373
DOI https://doi.org/10.36948/ijfmr.2024.v06i05.28373
Short DOI https://doi.org/g8pnrj
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